Kalafo e hlahang ea autologous cell e sebelisang plasma e ruileng ea platelet (PRP) e ka bapala karolo e thusang mererong e fapaneng ea kalafo ea kalafo e nchafatsang.Ho na le tlhoko e sa fihlellehang lefatšeng ka bophara ea maano a ho lokisa lisele bakeng sa ho phekola bakuli ba nang le mafu a mesifa le masapo a mokokotlo (MSK) le mafu a mokokotlo, lefu la masapo a manonyeletso (OA) le maqeba a sa foleng a rarahaneng le a refractory.Phekolo ea PRP e itšetlehile ka 'nete ea hore platelet growth factor (PGF) e tšehetsa pholiso ea maqeba le ho lokisa cascade (ho ruruha, ho ata le ho tsosolosoa).Ho 'nile ha hlahlojoa mefuta e mengata e fapaneng ea li-PRP ho tsoa lithutong tsa batho, tsa in vitro le tsa liphoofolo.Leha ho le joalo, likhothaletso tsa lithuto tsa in vitro le tsa liphoofolo hangata li lebisa liphethong tse fapaneng tsa kliniki, hobane ho thata ho fetolela sephetho sa lipatlisiso tse seng tsa tliliniki le likhothaletso tsa mekhoa kalafong ea batho.Lilemong tsa morao tjena, ho entsoe tsoelo-pele ea ho utloisisa mohopolo oa theknoloji ea PRP le baemeli ba likokoana-hloko, 'me litaelo tse ncha tsa lipatlisiso le lipontšo tse ncha li hlahisitsoe.Tlhahlobisong ena, re tla tšohla tsoelo-pele ea morao-rao ho lokiseng le ho hlophisoa ha PRP, ho kenyelletsa le tekanyo ea platelet, ts'ebetso ea leukocyte le tsamaiso ea 'mele ea ho itšireletsa mafung ea tlhaho le e feto-fetohang, phello ea 5-hydroxytryptamine (5-HT) le phomolo ea bohloko.Ho phaella moo, re buisane ka mochine oa PRP o amanang le ho ruruha le angiogenesis nakong ea ho lokisoa le ho tsosolosoa ha lisele.Qetellong, re tla hlahloba liphello tsa lithethefatsi tse ling mosebetsing oa PRP.
Autologous platelet-rich plasma (PRP) ke karolo ea metsi ea mali a autologous peripheral ka mor'a phekolo, 'me mahloriso a platelet a phahame ho feta a motheo.Phekolo ea PRP e 'nile ea sebelisoa bakeng sa lipontšo tse sa tšoaneng bakeng sa lilemo tse fetang 30, e leng se etsang hore ho be le thahasello e kholo ho bokhoni ba PRP e ikemetseng ka meriana e tsosolosang.Lentsoe "orthopedic biological agent" le sa tsoa hlahisoa ho phekola mafu a mesifa le masapo (MSK), 'me le fihletse liphello tse tšepisang bokhoni ba ho tsosolosoa ha metsoako ea lisele tsa PRP tsa heterogeneous bioactive.Hona joale, phekolo ea PRP ke khetho e nepahetseng ea phekolo e nang le melemo ea bongaka, 'me liphello tsa mokuli tse tlalehiloeng lia khothatsa.Leha ho le joalo, ho se lumellane ha liphello tsa mokuli le kutloisiso e ncha ho hlahisitse mathata ho ts'ebetsong ea ts'ebeliso ea kliniki ea PRP.E 'ngoe ea mabaka e ka' na ea e-ba palo le phapang ea mekhoa ea mofuta oa PRP le PRP 'marakeng.Lisebelisoa tsena li fapane ho ea ka palo ea pokello ea PRP le morero oa ho lokisetsa, o hlahisang litšobotsi tse ikhethang tsa PRP le li-agent tsa likokoana-hloko.Ho phaella moo, ho hloka tumellano mabapi le ho lekanngoa ha morero oa ho lokisetsa PRP le tlaleho e feletseng ea baemeli ba likokoana-hloko ka kopo ea bongaka e lebisitse ho liphello tse sa tsitsang tsa tlaleho.Boiteko bo bongata bo entsoe ho khetholla le ho arola PRP kapa lihlahisoa tse nkiloeng ka mali lits'ebetsong tsa meriana e nchafatsang.Holim'a moo, li-derivatives tsa platelet, joalo ka li- platelet lysate tsa motho, li entsoe tlhahiso bakeng sa lipatlisiso tsa lisele tsa 'mele le tsa in vitro.
E 'ngoe ea litlhaloso tsa pele ho PRP e ile ea hatisoa ka 2006. Sepheo se seholo sa tlhahlobo ena ke mosebetsi le mokhoa oa liketso tsa liplatelete, phello ea PRP sethaleng se seng le se seng sa pholiso ea pholiso, le karolo ea mantlha ea khōlo e entsoeng ke platelet. ka matšoao a fapaneng a PRP.Qalong ea lipatlisiso tsa PRP, thahasello e kholo ho PRP kapa PRP-gel e ne e le ho ba teng le mesebetsi e tobileng ea lintlha tse 'maloa tsa kholo ea platelet (PGF).
Leqepheng lena, re tla tšohla ka ho pharaletseng nts'etsopele ea morao-rao ea likarolo tse fapaneng tsa PRP le li-receptor tsa lisele tsa platelet le liphello tsa tsona ho laola tsamaiso ea 'mele ea ho itšireletsa mafung.Ho phaella moo, karolo ea lisele tsa motho ka mong tse ka 'nang tsa e-ba teng ka pitseng ea phekolo ea PRP le tšusumetso ea tsona ts'ebetsong ea ho tsosolosoa ha lisele li tla tšohloa ka ho qaqileng.Ho phaella moo, tsoelo-pele ea morao-rao ea ho utloisisa mahlahana a likokoana-hloko tsa PRP, tekanyo ea platelet, liphello tse khethehileng tsa lisele tse tšoeu tsa mali, le liphello tsa mahloriso a PGF le li-cytokine liphellong tsa phepo ea lisele tsa mesenchymal stem (MSCs) li tla hlalosoa, ho kenyelletsa le PRP e lebisang tlhokomelo e fapaneng. tikoloho ea lisele le lisele ka mor'a phetisetso ea lets'oao la sele le litlamorao tsa paracrine.Ka ho tšoanang, re tla tšohla mokhoa oa PRP o amanang le ho ruruha le angiogenesis nakong ea ho lokisoa le ho tsosolosoa ha lisele.Qetellong, re tla hlahloba phello ea analgesic ea PRP, phello ea lithethefatsi tse ling mosebetsing oa PRP, le motsoako oa PRP le mananeo a ho tsosolosa.
Melao-motheo ea mantlha ea kalafo ea plasma e nang le platelet e ngata
Litokisetso tsa PRP li ntse li tsebahala haholo ebile li sebelisoa haholo mafapheng a fapaneng a bongaka.Molao-motheo oa motheo oa saense oa phekolo ea PRP ke hore ho kenngoa ha liplatelete tse tsepamisitsoeng sebakeng se lemetseng ho ka qalisa ho lokisoa ha lisele, ho qaptjoa ha lisele tse ncha tse kopanyang le ho tsosolosoa ha phallo ea mali ka ho lokolla lintho tse ngata tse sebetsang ka biologically (lintho tsa kholo, cytokines, lysosomes) le liprotheine tsa ho khomarela tse ikarabellang bakeng sa ho qala karabelo ea hemostatic cascade.Ho phaella moo, liprotheine tsa plasma (mohlala, fibrinogen, prothrombin, le fibronectin) li teng likarolong tsa plasma tse futsanehileng tsa platelet (PPPs).Ho tsepamisa mohopolo oa PRP ho ka susumetsa ho lokolloa ha hyperphysiological ea lintlha tsa kholo ho qala pholiso ea kotsi e sa foleng le ho potlakisa mokhoa oa ho lokisa kotsi e mpe.Mehatong eohle ea ts'ebetso ea ho lokisoa ha lisele, lintlha tse fapaneng tsa kholo, li-cytokine le li-regulator tsa ts'ebetso ea lehae li khothaletsa mesebetsi e mengata ea mantlha ea lisele ka mekhoa ea endocrine, paracrine, autocrine le endocrine.Melemo e ka sehloohong ea PRP e kenyeletsa ts'ireletso ea eona le thekenoloji e bohlale ea ho lokisetsa thepa ea hona joale ea khoebo, e ka sebelisoang ho lokisetsa lisebelisoa tsa likokoana-hloko tse ka sebelisoang haholo.Habohlokoa ka ho fetisisa, ha ho bapisoa le corticosteroids e tloaelehileng, PRP ke sehlahisoa se ikemetseng se se nang litla-morao tse tsejoang.Leha ho le joalo, ha ho na taolo e hlakileng mabapi le sebopeho le sebopeho sa sebopeho sa PRP se nang le injectable, 'me ho hlophisoa ha PRP ho na le liphetoho tse kholo ho li-platelet, lisele tse tšoeu tsa mali (WBC), tšilafalo ea lisele tse khubelu tsa mali (RBC) le mahloriso a PGF.
Mareo a PRP le ho arola
Ka lilemo tse mashome, nts'etsopele ea lihlahisoa tsa PRP tse sebelisetsoang ho khothalletsa ho lokisoa le ho tsosolosoa ha lisele e 'nile ea e-ba karolo ea bohlokoa ea lipatlisiso tsa biomaterials le saense ea lithethefatsi.Thepa ea pholiso ea lisele e kenyelletsa barupeluoa ba bangata, ho kenyeletsoa liplatelete le lintlha tsa kholo ea bona le li-granules tsa cytokine, lisele tse tšoeu tsa mali, matrix a fibrin le li-cytokine tse ling tse ngata tsa synergistic.Ts'ebetsong ena ea cascade, ts'ebetso e rarahaneng ea coagulation e tla etsahala, ho kenyelletsa ts'ebetso ea platelet le densification e latelang le α- Ho lokolloa ha likahare tsa likaroloana tsa liplatelete, ho kopana ha fibrinogen (e lokollotsoeng ke liplatelete kapa mahala ho plasma) ho marang-rang a fibrin, le sebopeho. ho ruruha ha platelet embolism.
"Universal" PRP e etsisa tšimoloho ea pholiso
Qalong, lentsoe “platelet-rich plasma (PRP)” le ne le bitsoa platelet concentrate le sebelisoang phekolong ea tšelo ea mali, ’me le ntse le sebelisoa le kajeno.Qalong, lihlahisoa tsena tsa PRP li ne li sebelisoa feela e le sekhomaretsi sa lithane tsa fibrin, ha liplatelete li ne li sebelisoa feela ho tšehetsa polymerization e matla ea fibrin ho ntlafatsa ho tiisoa ha lisele, ho e-na le ho folisa.Ka mor'a moo, theknoloji ea PRP e ne e etselitsoe ho etsisa ho qalisoa ha pholiso ea pholiso.Ka mor'a moo, theknoloji ea PRP e ile ea akaretsoa ka bokhoni ba eona ba ho hlahisa le ho lokolla lintlha tsa kholo ho microenvironment ea lehae.Cheseho ena ea ho fana ka PGF hangata e pata karolo ea bohlokoa ea likarolo tse ling linthong tsena tse tsoang maling.Cheseho ena e matlafatsoa le ho feta ka lebaka la khaello ea lintlha tsa saense, litumelo tse makatsang, lithahasello tsa khoebo le khaello ea maemo le maemo.
Biology ea PRP concentrate e rarahane joaloka mali ka boeona, 'me e ka ba e rarahaneng ho feta lithethefatsi tsa setso.Lihlahisoa tsa PRP ke li-biomaterials tse phelang.Liphello tsa ts'ebeliso ea PRP ea kliniki li itšetlehile ka litšobotsi tse ka hare, tsa bokahohle le tse feto-fetohang tsa mali a mokuli, ho kenyelletsa le likarolo tse ling tse sa tšoaneng tsa lisele tse ka 'nang tsa e-ba teng sampuling ea PRP le microenvironment ea sebaka sa receptor, e ka bang boemong bo boima kapa bo sa foleng.
Kakaretso ea mantsoe a ferekanyang a PRP le tsamaiso e hlophisitsoeng ea lihlopha
Ka lilemo tse ngata, litsebi, bo-rasaense le lik'hamphani li 'nile tsa hlaseloa ke ho se utloisisane ha pele le mefokolo ea lihlahisoa tsa PRP le mantsoe a tsona a fapaneng.Bangoli ba bang ba hlalositse PRP e le platelet-feela, ha ba bang ba bontša hore PRP e boetse e na le lisele tse khubelu tsa mali, lisele tse sa tšoaneng tse tšoeu tsa mali, fibrin le liprotheine tsa bioactive tse nang le mahloriso a eketsehileng.Ka hona, li-agent tse ngata tse fapaneng tsa likokoana-hloko tsa PRP li kentsoe tšebetsong ea bongaka.Hoa soabisa hore ebe lingoliloeng hangata ha li na tlhaloso e qaqileng ea lintho tse phelang.Ho hloleha ha maemo a ho lokisoa ha lihlahisoa le nts'etsopele ea tsamaiso ea likarolo tse latelang e ile ea lebisa ho sebelisoeng ha palo e kholo ea lihlahisoa tsa PRP tse hlalosoang ka mantsoe le khutsufatso e fapaneng.Ha ho makatse hore ebe liphetoho tsa litokisetso tsa PRP li lebisa liphellong tse sa tsitsang tsa mokuli.
Kingsley o ile a qala ho sebelisa lentsoe "platelet-rich plasma" ka 1954. Lilemo tse ngata hamorao, Ehrenfest et al.Tsamaiso ea pele ea lihlopha tse thehiloeng ho mefuta e meraro e meholo (platelet, leukocyte le fibrin content) e ile ea etsoa, 'me lihlahisoa tse ngata tsa PRP li arotsoe ka lihlopha tse' nè: P-PRP, LR-PRP, fiber e hloekileng ea platelet-rich (P-PRF) le leucocyte. PRF e ruileng (L-PRF).Lihlahisoa tsena li lokisoa ka sistimi e koetsoeng ka botlalo kapa protocol ea letsoho.Ho sa le joalo, Everts et al.Bohlokoa ba ho bua ka lisele tse tšoeu tsa mali litokisetsong tsa PRP bo ile ba hatisoa.Ba boetse ba khothaletsa tšebeliso ea mantsoe a nepahetseng ho bolela mefuta e sa sebetseng kapa e kentsoeng ea litokisetso tsa PRP le gel ea platelet.
Delong et al.e hlahisitse tsamaiso ea PRP e bitsoang li-platelet, lisele tse tšoeu tsa mali tse sebetsang (PAW) tse thehiloeng ho palo e feletseng ea li-platelet, ho kenyelletsa le mekhahlelo e mene ea li-platelet.Mekhahlelo e meng e kenyelletsa tšebeliso ea li-activator tsa platelet le ho ba teng kapa ho ba sieo ha lisele tse tšoeu tsa mali (ke hore li-neutrophils).Mishra et al.Ho hlahisoa mokhoa o tšoanang oa ho arola.Lilemo tse 'maloa hamorao, Mautner le basebetsi-'moho le eena ba ile ba hlalosa tsamaiso e hlakileng le e qaqileng ea lihlopha (PLRA).Sengoli se pakile hore ho bohlokoa ho hlalosa palo e felletseng ea liplatelete, lisele tse tšoeu tsa mali (tse ntle kapa tse mpe), liperesente tsa neutrophil, RBC (tse ntle kapa tse mpe) le hore na ts'ebetso ea kantle ho naha e sebelisoa.Ka 2016, Magalon et al.Sehlopha sa DEPA se ipapisitse le tekanyo ea ente ea platelet, katleho ea tlhahiso, bohloeki ba PRP e fumanoeng le ts'ebetso ea ts'ebetso e ile ea hatisoa.Ka mor'a moo, Lana le basebetsi-'moho le eena ba ile ba hlahisa mokhoa oa ho arola MARSPILL, o shebane le lisele tsa "mononuclear" tsa mali.Haufinyane tjena, Komiti ea Boemo ba Saense e ile ea buella tšebeliso ea tsamaiso ea lihlopha tsa Mokhatlo oa Machaba oa Thrombosis le Hemostasis, e thehiloeng letotong la likhothaletso tsa tumellano ea ho tiisa tšebeliso ea lihlahisoa tsa platelet lits'ebetsong tsa meriana e nchafatsang, ho kenyeletsoa le lihlahisoa tsa liplatelete tse leqhoa le tse qhibilihisitsoeng.
Ho itšetlehile ka tsamaiso ea PRP e hlophisitsoeng ke litsebi tse fapa-fapaneng le bafuputsi, boiteko bo bongata bo sa atleheng ba ho leka-lekanya tlhahiso, tlhaloso le foromo ea PRP e lokelang ho sebelisoa ke lingaka e ka fihlela qeto e nepahetseng, e ka 'nang ea se ke ea etsahala lilemong tse' maloa tse latelang Ho phaella moo. , thekenoloji ea lihlahisoa tsa PRP ea kliniki e ntse e tsoela pele, 'me boitsebiso ba saense bo bontša hore ho hlokahala litokisetso tse fapaneng tsa PRP ho phekola mafu a sa tšoaneng tlas'a maemo a itseng.Ka hona, re lebeletse hore mekhahlelo le mefuta-futa ea tlhahiso e ntle ea PRP e tla tsoela pele ho hōla nakong e tlang.
Mokhoa oa ho lokisetsa PRP o ntse o tsoela pele
Ho ea ka poleloana ea PRP le tlhaloso ea sehlahisoa, mekhoa e mengata ea lihlopha e lokolloa bakeng sa mekhoa e fapaneng ea PRP.Ka bomalimabe, ha ho na tumellano mabapi le tsamaiso e pharaletseng ea lihlopha tsa PRP kapa mali leha e le afe a autologous le lihlahisoa tsa mali.Ka mokhoa o loketseng, tsamaiso ea lihlopha e lokela ho ela hloko litšobotsi tse sa tšoaneng tsa PRP, litlhaloso le nomenclature e nepahetseng e amanang le liqeto tsa phekolo ea bakuli ba nang le mafu a itseng.Hona joale, lits'ebetso tsa masapo li arola PRP ka mekhahlelo e meraro: pure platelet-rich fibrin (P-PRF), leucocyte-rich PRP (LR-PRP) le leucocyte-deficient PRP (LP-PRP).Le hoja e tobile ho feta tlhaloso e akaretsang ea sehlahisoa sa PRP, lihlopha tsa LR-PRP le LP-PRP ho hlakile hore ha li na boitsebiso bo itseng ka har'a lisele tse tšoeu tsa mali.Ka lebaka la mekhoa ea eona ea ho itšireletsa mafung le ho itšireletsa, lisele tse tšoeu tsa mali li amme haholo biology ea mantlha ea mafu a sa foleng a lisele.Ka hona, li-biological agents tsa PRP tse nang le lisele tse tšoeu tsa mali tse khethehileng li ka khothalletsa haholo taolo ea 'mele ea ho itšireletsa mafung le ho lokisoa ha lisele le ho tsosolosoa.Haholo-holo, li-lymphocyte li ngata ho PRP, li hlahisa insulin-like growth factor le ho tšehetsa phetoho ea lisele.
Li-monocyte le macrophages li bapala karolo ea bohlokoa ts'ebetsong ea taolo ea 'mele ea ho itšireletsa mafung le mokhoa oa ho lokisa lisele.Bohlokoa ba li-neutrophils ho PRP ha bo hlake.LP-PRP e ile ea khethoa e le tokisetso ea pele ea PRP ka tlhahlobo e hlophisitsoeng ho finyella liphello tse sebetsang tsa phekolo ea OA e kopanetsoeng.Leha ho le joalo, Lana et al.Tšebeliso ea LP-PRP ho phekola OA ea lengole e hanyetsanoa, e leng se bontšang hore lisele tse tšoeu tsa mali tse khethehileng li phetha karolo ea bohlokoa ts'ebetsong ea ho ruruha pele ho tsosolosoa ha lisele, hobane li lokolla limolek'hule tsa pro-inflammatory le anti-inflammatory.Ba fumane hore motsoako oa li-neutrophils le liplatelete tse kentsoeng tšebetsong li na le litlamorao tse ntle ho feta litlamorao tse mpe ho lokisoeng ha lisele.Ba boetse ba bonts'a hore polasetiki ea monocyte ke ea bohlokoa bakeng sa ts'ebetso e se nang ho ruruha le ho lokisoa ho lokisoeng ha lisele.
Tlaleho ea morero oa ho itokisa oa PRP lipatlisisong tsa kliniki ha e lumellane haholo.Lithuto tse ngata tse hatisitsoeng ha lia hlahisa mokhoa oa ho itokisa oa PRP o hlokahalang bakeng sa ho pheta-pheta ha morero.Ha ho na tumellano e hlakileng har'a lipontšo tsa phekolo, kahoo ho thata ho bapisa lihlahisoa tsa PRP le liphello tsa tsona tse amanang le phekolo.Maemong a mangata a tlalehiloeng, phekolo ea mahloriso ea platelet e arotsoe tlas'a lentsoe "PRP", esita le bakeng sa pontšo e tšoanang ea kliniki.Bakeng sa likarolo tse ling tsa bongaka (tse kang OA le tendinosis), tsoelo-pele e entsoe ho utloisisa liphetoho tsa litokisetso tsa PRP, litsela tsa ho pepa, mosebetsi oa platelet le likarolo tse ling tsa PRP tse amang ho lokisoa ha lisele le ho tsosolosoa ha lisele.Leha ho le joalo, lipatlisiso tse ling lia hlokahala ho fihlela tumellano mabapi le poleloana ea PRP e amanang le mahlahana a likokoana-hloko a PRP e le hore ho phekoloe ka botlalo le ka mokhoa o sireletsehileng mafu a itseng le maloetse.
Boemo ba tsamaiso ea lihlopha tsa PRP
Tšebeliso ea li-autologous PRP biotherapy e tšoenngoa ke ho fapana ha litokisetso tsa PRP, ho reha mabitso a sa tsitsang le maemo a fokolang a tataiso e thehiloeng bopaking (ke hore, ho na le mekhoa e mengata ea ho itokisa ho hlahisa lijana tsa phekolo ea meriana).Ho ka boleloa esale pele hore litaba tsa PRP tse felletseng, bohloeki le litšobotsi tsa tlhaho tsa PRP le lihlahisoa tse amanang le tsona li fapana haholo, 'me li ama katleho ea likokoana-hloko le liphetho tsa liteko tsa kliniki.Khetho ea sesebelisoa sa ho lokisetsa PRP e hlahisa phapang ea pele ea bohlokoa.Meriana ea ho tsosolosa ea kliniki, lingaka li ka sebelisa lisebelisoa tse peli tse fapaneng tsa ho lokisetsa PRP le mekhoa.Tokisetso e sebelisa mochine o tloaelehileng oa ho arola lisele tsa mali, o sebetsang maling a feletseng a bokeletsoeng ka bobona.Mokhoa ona o sebelisa moqomo o tsoelang pele oa phallo ea centrifuge kapa thekenoloji ea karohano ea disk le mehato e thata le e bonolo ea centrifuge.Boholo ba lisebelisoa tsena li sebelisoa ha ho buuoa.Mokhoa o mong ke oa ho sebelisa theknoloji ea centrifugal ea matla a khoheli le lisebelisoa.High G-force centrifugation e sebelisoa ho arola lera le mosehla la ESR ho tloha yuniti ea mali e nang le liplatelete le lisele tse tšoeu tsa mali.Lisebelisoa tsena tsa mahloriso li nyane ho feta tse arolang lisele tsa mali 'me li ka sebelisoa haufi le bethe.Phapang ģ - Nako ea matla le centrifugation e lebisa phapanong e kholo ho chai, ho tsepamisa maikutlo, ho hloeka, ho sebetsa, le boemo ba ts'ebetso ea liplatelete tse ka thoko.Mefuta e mengata ea thepa ea ho lokisetsa PRP ea khoebo e ka sebelisoa sehlopheng sa ho qetela, e leng se bakang liphetoho tse ling tsa lihlahisoa tsa lihlahisoa.
Ho hloka tumellano mabapi le mokhoa oa ho itokisa le ho netefatsa PRP ho ntse ho tsoela pele ho lebisa ho se lumellane ha phekolo ea PRP, 'me ho na le phapang e khōlō ea ho lokisetsa PRP, boleng ba mohlala le liphello tsa kliniki.Thepa e teng ea khoebo ea PRP e netefalitsoe mme e ngolisitsoe ho ea ka litlhaloso tsa moetsi oa thepa, e leng se rarollang mefuta e sa tšoaneng har'a lisebelisoa tsa PRP tse teng hona joale.
Utloisisa tekanyo ea platelet in vitro le in vivo
Phello ea phekolo ea PRP le platelet e 'ngoe e tsepamisitse maikutlo ho tsoa ho ho lokolloa ha lintlha tse sa tšoaneng tse amehang ho lokisoeng ha lisele le ho tsosolosoa.Ka mor'a ts'ebetso ea li-platelet, li-platelet li tla theha platelet thrombus, e tla sebetsa e le matrix ea nakoana ea extracellular ho khothalletsa ho ata ha lisele le ho arohana.Ka hona, hoa utloahala ho nahana hore tekanyo e phahameng ea platelet e tla lebisa tlhokomelong e phahameng ea libaka tsa platelet bioactive factor.Leha ho le joalo, kamano pakeng tsa lethal dose le mahloriso a liplatelete le mahloriso a ho lokolloa ha platelet bioactive growth factor le lithethefatsi e ka 'na ea se ke ea laoleha, hobane ho na le phapang e kholo ea palo ea motheo ea platelet pakeng tsa bakuli ka bomong,' me ho na le phapang pakeng tsa mekhoa ea ho lokisetsa PRP.Ka mokhoa o ts'oanang, lintlha tse 'maloa tsa ho hōla ha platelet tse amehang mochineng oa ho lokisa lisele li teng karolong ea plasma ea PRP (mohlala, ntlha ea ho hōla ha sebete le insulin-like factor factor 1).Ka hona, tekanyo e phahameng ea platelet e ke ke ea ama bokhoni ba tokiso ea lintlha tsena tsa kholo.
Patlisiso ea in vitro PRP e tumme haholo hobane mekhahlelo e fapaneng lithutong tsena e ka laoloa ka nepo mme liphetho li ka fumanoa kapele.Liphuputso tse 'maloa li bontšitse hore lisele li arabela ho PRP ka mokhoa o itšetlehileng ka tekanyo.Nguyen le Pham ba bontšitse hore likhahla tse phahameng haholo tsa GF li ne li hlile li sa lumellane le ts'ebetso ea ho tsosoa ha lisele, e ka 'nang ea se ke ea e-ba molemo.Liphuputso tse ling tsa in vitro li bonts'itse hore khatello e phahameng ea PGF e ka ba le litlamorao tse mpe.Lebaka le leng e ka ba palo e lekanyelitsoeng ea li-receptor tsa membrane ea lisele.Ka hona, hang ha boemo ba PGF bo phahame haholo ha bo bapisoa le li-receptor tse teng, li tla ba le phello e mpe tšebetsong ea lisele.
Bohlokoa ba data ea mahloriso ea platelet in vitro
Leha lipatlisiso tsa in vitro li na le melemo e mengata, le tsona li na le mefokolo e itseng.In vitro, ka lebaka la tšebelisano e tsoelang pele lipakeng tsa mefuta e mengata e fapaneng ea lisele liseleng life kapa life ka lebaka la sebopeho sa lisele le lisele tsa lisele, ho thata ho pheta-pheta in vitro tikolohong ea setso se nang le likarolo tse peli.Boima ba lisele bo ka amang tsela ea matšoao a lisele hangata bo ka tlase ho 1% ea boemo ba lisele.Lisele tse nang le mahlakore a mabeli li thibela lisele ho pepesehela matrix a extracellular (ECM).Ho feta moo, theknoloji e tloaelehileng ea setso e tla lebisa ho bokelleng litšila tsa lisele le tšebeliso e tsoelang pele ea limatlafatsi.Ka hona, setso sa in vitro se fapane le boemo leha e le bofe bo tsitsitseng, phepelo ea oksijene ea lisele kapa phapanyetsano ea tšohanyetso ea setso, 'me liphello tse hanyetsanang li hatisitsoe, ho bapisa phello ea kliniki ea PRP le thuto ea in vitro ea lisele tse itseng, mefuta ea lisele le platelet. mahloriso.Graziani le ba bang.Ho ile ha fumanoa hore in vitro, phello e kholo ka ho fetisisa ea ho ata ha osteoblasts le fibroblasts e ile ea finyelloa ho PRP platelet concentrations 2.5 linako tse phahameng ho feta boleng ba motheo.Ka lehlakoreng le leng, lintlha tsa kliniki tse fanoeng ke Park le basebetsi-'moho li bontšitse hore ka mor'a ho kopana ha mokokotlo, boemo ba platelet ba PRP bo hloka ho eketseha ka makhetlo a fetang 5 ho feta motheo oa ho hlahisa liphello tse ntle.Liphetho tse tšoanang tse hanyetsanang li ile tsa boela tsa tlalehoa pakeng tsa data ea ho ata ha tendon in vitro le liphello tsa kliniki.
(Likahare tsa sengoloa sena li hatisitsoe hape, 'me ha re fane ka tiisetso efe kapa efe e hlakileng kapa e boletsoeng bakeng sa ho nepahala, ho ts'epahala kapa botlalo ba litaba tse teng sengolong sena,' me ha re ikarabelle bakeng sa maikutlo a sengoloa sena, ka kopo utloisisa.)
Nako ea poso: Mar-01-2023